Jul 17, 2019 the gene pulser xcell total system is the complete electroporation system for transfection of both eukaryotic and prokaryotic cells. Owners manuals and users guides for accessories for water bio rad gene pulser electroporation buffer. These transfection instruments are cited extensively in the. Nuclease 3nls complexed with altr crisprcas9 crrna and tracrrna in the presence of altr cas9 electroporation enhancer, on the amaxa 4d and bio rad gene pulser platforms. Gene pulser xcell an exceptional system for excellent results the gen. The gene pulser xcell is a modular system, comprising of a main unit and two accessory modules, the ce module and the pc module, in addition to the shocking chamber and a cuvette with incorporated electrodes. Es cell targeting using the bio rad gene pulser ii modified from introduction of foreign dna into embryonic stem cells, chapter 10, in manipulating the mouse embryo, 3rd edition, by andras nagy, marina gertsenstein, kristina vintersten, and richard behringer. Bio rad cfx96 instruction manual pdf download manualslib. What electroporation settings to use for li k12 in a 0. For technical assistance contact your local biorad office or, in the us. If any defects occur in the instrument during this warranty period, biorad laboratories will, at bio rad s option, repair or replace the defective parts free of charge. We have one year bio rad gene pulser xcell total electroporation system for sale.
Call 18004biorad 18004246723 gene pulser ii electroporation system instruction manual. Gibson assembly sitedirected mutagenesis kit instructions. The bio rad gene pulsar xcell electroporation system is a pulse generator that uses capacitors to produce controlled exponential or square wave electrical pulses for cell electroporation. Biorad laboratories is not responsible for any injury or damage caused by the use of this instrument for purposes other than those for which it is intended, or by. Gene pulser ii electroporation system instruction manual. Visit our website click here artisan technology group. A modular pulse generator system used for electroporation of a wide variety of. Gene pulser ii instruction manual 165xxxx series rev c pdf. Place the cell suspension in the gene pulser cuvette. The cells were transferred to electroporation cuvettes 2 mm, bio rad, pulsed at v jurkat, v frhk4, and v thp1 and u monocytes using a bio rad model gene pulser xcell system, following the manual to select conditions.
It consists of the gene pulser ii, capacitance extender plus, and pulse controller plus. This drop in voltage is called the pulse droop and it. Bio rad life science research 2015 product catalog. Performing sitedirected mutagenesis of the control plasmid with control primer mixes. Place 15 l of dna in a sterile microcentrifuge tube and chill it on ice. The biorad gene pulser xcell system is a flexible, modular electroporation system that can transfect all cell types, from primary and stem cells to bacteria and yeast. Cuvette volume each cuvette can contain up to 100 l of cells plus 1 ml of media. This strain has the wild type enda gene and requires the additional wash step when using commercial plasmid purification kits. Gibson assembly ultra kit instructions catalog numbers ga1200s, ga120010, ga120050, ga120010mm, ga120050mm rev. Aineen tai seoksen merkitykselliset tunnistetut kaytot ja kaytot, joita ei suositella kayttotarkoitus laboratoriokemikaalit 1. L contains an ampicillin resistance gene ampr and a mutated lacz gene, which allows for bluewhite screening of colonies on lb agar plates containing 100. The ce module is recommended for use with the gene pulser xcell main unit for electroporation of most.
This will ensure lyophilised primer and probe mix is in the base of the tube and is not lost upon opening the tube. Instruments, including the gene pulser series of electroporators, the micropulser electroporator, and the helios gene gun and pdshe biolistic systems, have been used successfully to transfect prokaryotic and eukaryotic cells, including plant and animal cells. Biorad gene pulser xcell eukaryotic electroporation system. Cold spring harbor laboratory press, cold spring harbor, ny, usa, 2003. Detailed info and photos for this can be found on our website at bio rad gene pulser xcelleukaryoticelectroporationsystem16526. Bio rad helios gene gun system instruction manual pdf. The helios gene gun the helios gene gun is the second instrument in bio rad s particle delivery product line. The pulse controller unit is used with the gene pulser apparatus for the electroporation of bacteria, or for other applications where pulses of very. Bio rad pcr pictorial guide 111011 university of florida. Biorad laboratories warrants the micropulser against defects in materials and workmanship for 1 year. Robust genome editing achieved using electroporation with the bio rad gene pulser xcell system. Consult biorads entire electroporation systems catalogue on medicalexpo. Electroporation systems biorad pdf catalogs technical. Read online or download pdf page 82 83 biorad gene pulser xcell electroporation systems user manual biorad accessories for water.
We providing 1 pdf manuals bio rad gene pulser electroporation buffer for download free by document types. Thaw the soc medium to add to the cells after the pulse. In addition, the gene pulser ii system is the only electroporation device that accurately reports the actual time constant of every pulse, so that your protocols can be. View and download bio rad cfx96 instruction manual online. After pulse, place cells and dna in recovery media enriched media. Resuspend the primerprobe mix in the rnasednase free water supplied, according to the table below. Schwer lab es cell targeting using the biorad gene pulser ii. High efficiency crispr gene editing with biorads gene. Experiments were performed in triplicate on three separate days and the data averaged. Twentyfour hours postelectroporation, cells were assayed for viablility by propidium iodide staining and flow cytometry analysis. Gene pulser ii rf module instruction manual american. Biorad gene pulser xcell electroporation system youtube. Biorad electroporator gene pulser xcell psc 543 micropulser. Tuotteen nimi gene pulser electroporation buffer luettelonumerot 1652676, 9179, 1652677 puhdas aineseos seos 1.
Compatible with any electroporation buffer, including bio rad s gene pulser electroporation buffer. The rf gene pulser module, the latest addition to the gene pulser ii electroporation family, permits the user to electroporate samples using either square or sinusoidal waves. Electroporator suitable for electroporation of bacterial cells, e. Provides a choice of programs for manual operation, preset protocols, user protocols, and an optimization protocol. Gene transfer gene pulser xcell electroporation system biorad. Section 6 and the pulse controller manual for a detailed discussion of this effect. Introducing dna and rna into cells is a powerful tool for evaluating gene. Manual program capability allows voltage to be selected in a 2003,000 v range with 10 v precision, and the pulse width to be selected in a 1. If using the bio rad gene pulser unit, use the following conditions to get efficiencies of 1. For instructions for doing these 2 please use manual. Transfection apparatus operating instructions and applications guide version 1090 for technical service call 18004biorad 18004246723. For technical service call your local bio rad office or in the u.
Gene pulser xcell an exceptional system for excellent results the gene pulser xcell is a flexible, modular pulse delivery system that uses exponential or squarewave pulses to deliver the pulses optimal for your cell type. Wash several times and spin several times 5 10 min each. Gene pulser mxcell electroporation system flier, rev b. Instruction manual bio rad the back of this manual. You can also modify a preset protocol and save it under user protocols. In contrast to the pdshe system, in which the overall size of the target to be transformed is limited by the size of the chamber, and the target tissue is subjected to a vacuum during bombardment, the helios gene gun requires no. Bio rad and gene pulser are registered trademarks and micropulser is a trademark. The unit is capable of producing pulses of up to 3000v on a highvoltage circuit, and up to 500v on a lowvoltage circuit. Gene pulser micropulser cuvettes 1652083 pkg of 5, 0.
The following defects, however, are specifically excluded. Electroporation is a process whereby the membrane of a cell is temporarily destabilized in specific regions by exposure to high intensity electric field pulses. Biorad gene pulser xcell electroporation systems user manual. Cfx96 touch realtime pcr detection system brochure biorad. Artisan technology group is your source for quality new and certifiedusedpreowned equipment fast. Bio rad gene pulser xcell electroporation system is available from the lab world group. Gene pulser xcell systems 1652660 gene pulser xcell total system, for mammalian and microbial cells, 100240 v, 5060 hz, exponential and squarewave delivery, includes main unit, ce module, pc module, shockpod chamber, 15 sterile cuvettes 5 each of 0. Bio rad gene pulser electroporation buffer manuals. To ensure complete resuspension, vortex the tube thoroughly. Read the instruction manual before using the gene pulser xcell electroporation system. The pulse controller unit catalog number 1652098 is used with the gene pulser apparatus for electroporation of bacteria, fungi, or other applications where pulses of very high field strength are applied to samples of small volume and high resistance.
Bio rad gene pulser ii manual is a unique photo sharing app. See section 3 of the gene pulser manual for use of the capacitance extender. Calculation of transformation efficiency transformation efficiency te is defined as the number of colony forming units cfu produced by transforming 1g of plasmid into a given volume of competent cells. Pulse spin each tube in a centrifuge before opening.
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